Regression of devil facial tumour disease following immunotherapy in immunised Tasmanian devils

A new research paper shows evidence that immunization with devil facial tumor cells plus adjuvants (i.e. immune stimulators) primes anti-tumor immune responses. Subsequent booster shots with live tumor cells induced tumor regressions in 3/5 devils.

The first Wild Immunity article about our checkpoint molecule research in Tasmanian devils is published in The Conversation

Wild Immunity research about immune checkpoint molecules is published in Frontiers in Immunology

This new publication shows that the key immune checkpoint molecule PD-L1 (aka B7-H1) is upregulated on devil facial tumour cells in response to interferon-gamma (IFNg). This could be an important immune evasion mechanism used by the tumour to shut off anti-tumour responses by T cells and NK cells.

Wild Immunity research published in Functional Ecology is featured in Smithsonian magazine

Wild Immunity research published in Functional Ecology is featured in Smithsonian magazine.

Another contagious cancer discovered

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Check out the latest wild immunity research!

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PLOS ONE: Markedly Elevated Antibody Responses in Wild versus Captive Spotted Hyenas Show that Environmental and Ecological Factors Are Important Modulators of Immunity

Andrew S. Flies

Latest video about Tasmanian devil facial tumour

Check out a nice video from Smarter Every Day on the Tasmanian devil facial tumor disease. Most of the people in the video, except the host, are my new colleagues at the Menzies Research Institute Tasmania.

A complement to the ancient mariner

?w=660″ alt=”sponge1″ width=”660″ height=”495″ /> Washed up at Innes National Park. Photo by Andrew S. Flies

At first glance sponges may look like the most helpless animals on the planet, simply drifting with the ocean currents with no means of changing course or defending themselves. Sponges have no arms or legs for moving themselves around, no eyes or other obvious sense organs, and nothing that most people would consider a brain. Without any of the tools (i.e. big brains, opposable thumbs, bipedal walking, etc.) that helped modern humans spread across the planet, sponges have been floating around the earth for at least 500 million years.

One of the main themes of Wild Immunity is that every living thing on the planet needs to defend itself against exploitation. So how have the seemingly helpless waifs that we call sponges managed to defend themselves? One extremely effective defense system that sponges use is the complement system (reviewed by Leslie, 2012). No, they don’t whisper sweet compliments to their would-be parasites and convince the potential disease causing agents to attack something else. The complement system is set of proteins that work together to kill or disable pathogens. The complement system has proven so effective at neutralizing microorganisms, such as bacteria, that the system has been conserved across hundreds of millions of years of evolution, and there are clear similarities between complement proteins found in sponges and humans (reviewed by Leslie, 2012).

History

In the late 1800s it was discovered that blood serum could kill bacteria. The next discovery was that some of the bacterial-killing capacity of serum could be destroyed by heating the serum. The term complement was coined by Paul Ehrlich to describe this “heat-labile” component of serum. Since then, the system has been extensively studied and roughly 30 proteins have been identified that play a role in the mammalian complement system, and complement protein homologues (evolutionary-related proteins) have been found in nearly all animals, including sponges.

How it works

There are several different pathways (classical, alternative, and lectin-binding) that the complement system uses to kill bacteria, but all three pathways are dependent on the formation of a C3-convertase, which cleaves C3 into C3a and C3b and kicks-off of a cascade that cleaves other proteins into active forms. The end-product is a membrane attack complex formed by C5b, C6, C7, C8, and C9 that pokes a hole in the bacterial cell membrane, causing the bacterial cell to lose its ability to maintain homeostasis and die. Specialized immune cells called phagocytes then detect the complement proteins bound to the bacterial products and clean up the mess.

More than just a bacteria killer

Complement is such an effective and ubiquitous defender against microorganisms, that many pathogens have evolved proteins that attempt to disable complement. For example, the deadly variola virus (aka smallpox) produced proteins that bind to and disable C4b and C3b. In addition to complement prowess as a defender of homeostasis, over the past few decades it has become increasingly clear that complement is integrated into many aspects of immune defenses and general physiology. In addition to its important task of killing bacteria, it also helps neutralize viruses and parasitic worms, clears dead host cells, form new blood vessels, generate new tissue following injury, and direct formation of new neuronal synapses (Ricklin et al., 2010). Defects in complement genes have been associated with many diseases, such as cancer and lupus (Madelson, et al., 2004), and research is rapidly uncovering new roles for complement. The short version of this story is that the complement system has been critical for survival for millions of animals for millions of years, and still has important and diverse roles in health and disease today.

Question of the week

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More information

Simple animation of the complement pathways

Encyclopedia of Life: Sponges

References

Leslie, M., 2012. The New View of Complement. Science 337, 1034-1037.

Manderson, A.P., Botto, M., Walport, M.J., 2004. The role of complement in the development of systemic lupus erythematosus. Annu. Rev. Immunol. 22, 431–456

Ricklin, D., Hajishengallis, G., Yang, K., Lambris, J.D., 2010. Complement: a key system for immune surveillance and homeostasis. Nat Immunol 11, 785-797.