Resources

 

 

Immunology

Immuno-Navigator A database for gene coexpression in the immune system

The Biolgend web is an outstanding for all things immunology

CD molecules (Biolegend)

Cell markers (Biolegend)

Maturation markers (Biolegend)

Flow cytometry buffers (Biolegend)

Fluorescence spectra analyzer (Biolegend)

 

Cell Culture

Cell Counting with a Hemocytometer (BitesizeBio) – Click here to download PDF

Useful Numbers for Cell Culture (Thermo Scientific)

 

Microbiology and molecular biology links

Interactive molecular biology tools (NEB)

Molecular biology protocols (NEB)

Plasmid guide (Addgene) – very useful for cloning questions

Making LB agar plate – protocol  Making LB agar plate – video (Addgene)

Making glycerol stocks (Addgene)

Antibiotic concentrations spreadsheet (Addgene, InvivoGen)

Antibiotic concentrations (Thermo Fisher)

How to verify your plasmid  (Addgene)

Plasmids 101: The promotor region (Addgene) Download PDF

 

Protein analysis and prediction

Predict Eukaryotic Linear Motifs (ELM) (e.g. ITIM, ITSM, ITAM)

Predict protein structure and features: SMART (e.g. IG domains, signal peptides)

Predict protein domains and signal peptides: Phobius

Predict protein domains: TMHMM server

Predict signal peptides: SignalP

Predict molecular weight and other good stuff: Protparam ProtPI

Predict non-classically secreted proteins: SecretomeP

Calculate multi-protein percent identity: Clustal Omega

Predict nuclear localization signals: cNLS mapper

 

Recipes

General lab recipes (Use at your own risk)

50x TAE stock

 

Protocols

  1. CHO cell adaption to serum-free media
  2. Hybridoma production
  3. Hyrbidoma screening ELISA

 

Working with animals

Procedures with care – tutorial videos for animal techniques

 

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